26/03/2020
The Human Resources Strategy for Researchers

PhD contract in the field of Physics financed by the University Clermont Auvergne

This job offer has expired


  • ORGANISATION/COMPANY
    Université Clermont Auvergne
  • RESEARCH FIELD
    Physics
  • RESEARCHER PROFILE
    First Stage Researcher (R1)
  • APPLICATION DEADLINE
    21/05/2020 23:00 - Europe/Brussels
  • LOCATION
    France › AUBIERE
  • TYPE OF CONTRACT
    Other
  • JOB STATUS
    Other
  • HOURS PER WEEK
    35 H
  • OFFER STARTING DATE
    01/10/2020
  • REFERENCE NUMBER
    UCA/SF/0017
  • IS THE JOB RELATED TO STAFF POSITION WITHIN A RESEARCH INFRASTRUCTURE?
    Yes

Title of the thesis: Elucidate the mechanistic basis of mitochondrial disorders using single molecule biophysics tools

Supervisor : Emmanuel BUSATO

Laboratory : UMR CNRS 6533 - Laboratoire de Physique de Clermont (LPC)

University : UCA

Email and Phone : Emmanuel.BUSATO@uca.fr - 04 73 40 72 97

Possible co-supervisor : Géraldine Farge

Laboratory : UMR CNRS 6533 - Laboratoire de Physique de Clermont (LPC)

University : UCA

Summary :

Using a spectrum of single-molecule tools developed in our lab, we propose to unravel parts of the mechanochemistry and physical properties of mechanisms that are essential for cellular energy production.

Mitochondria are organelles responsible for the creation of cellular fuel (ATP). Strikingly, they contain their own DNA (mtDNA) which is reminiscent of bacterial DNA. This is not surprising, because mitochondria are the descendent of free living bacteria that took up residence into other cells. Once the symbiosis was established, genes required for a free-living lifestyle were deleted and most of the genes were transferred to the nucleus. The genes on this mitochondrial DNA are propagated (replication) by an enzymatic machinery (replisome) of fundamental importance, which can fine tune the cellular metabolism. DNA replication is an intricate molecular mechanism that is both fast and accurate. However, the physics underlying this impressive process, i.e. the connections between forces, motions and energy conversion, are largely unknown.

Single-molecule tools are in constant development and, as a result, increasingly complex biological systems can be visualized, controlled and measured. Human mitochondria are essential elements of complex eukaryotic cells; nevertheless many key components of this system can be reproduced in test tubes and so become accessible for biophysical techniques. The aim of this phD project is to investigate the mechanisms of human mtDNA replication using biophysical tools. We will use single-molecule instrumentation -namely Acoustic Force Spectroscopy (AFS) and TIRF microscopy- to visualize and exert forces on fluorescently-tagged proteins. These tools will allow the quantification of parameters such as binding/unbinding rates and forces of proteins on DNA, their speed, processivity, and direction as well as the DNA elastic properties. In addition, as alterations of mtDNA are linked to numerous health issues including aging, we will investigate how disease-causing mutations of replisome proteins can derail the replication machinery. Normal and dysfunctional proteins will be directly compared, permitting insight in the mechanistic basis of a disorder. Such knowledge will elucidate how robust mitochondrial replication is and how components need to interact to function properly.

Required knowlegde and skills: Theorical knowledge and experimental skills in biophysics (microscopy, single molecule manipulation)

Keywords: Single-Molecule Biophysics, Acoustic Force Spectroscopy, TIRF microscopy, Mitochondria, DNA-protein machinery

 

More informations via the link :

https://sf.ed.uca.fr/financement-doctoral/contrat-doctoral-allocations-ministerielles/sujets-de-these-ed-sf-2020-proposes-au-concours/

 

 

To apply, please download the application file via the link :

https://sf.ed.uca.fr/financement-doctoral/contrat-doctoral-allocations-m...

 
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Benefits

 

 
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Eligibility criteria

 
 
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Interested students must send two copies of their application to the secretariat of the doctoral school before 22 May 2020, the deadline.

Audition at the doctoral school of the selected candidates:

- 4 June morning: LAMP jury

- 11th June morning: LMV jury

- 11 June afternoon: Chemistry jury

- 12 June: Physics jury

19 June: ED SF council for allocations Doctoral allocations

 
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Offer Requirements

  • REQUIRED EDUCATION LEVEL
    Physics: Master Degree or equivalent

Skills/Qualifications

 
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Specific Requirements

Speak French or very good level of English

 
 
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Map Information

Job Work Location Personal Assistance locations
Work location(s)
1 position(s) available at
Clermont Laboratory of Physics (LPC)
France
Région Auvergne Rhône-Alpes
AUBIERE
63178
Campus Universitaire des Cézeaux TSA 60026 CS 60026 4, Avenue Blaise Pascal

Open, Transparent, Merit based Recruitment procedures of Researchers (OTM-R)

Know more about it at Université Clermont Auvergne

Know more about OTM-R

EURAXESS offer ID: 508404

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